The validated multiplex real-time PCR was used to detect and differentiate BoHV-1 and BoHV-5 in bovine clinical samples with known histories. The assay specificity was demonstrated by testing bacterial and viral DNA from pathogens commonly isolated from bovine respiratory and reproductive tracts. AlleleID® is a comprehensive desktop tool designed to address the challenges of bacterial identification, pathogen detection or species identification. Standard curves relating Ct value to template copy number had correlation coefficients of 0.989 for BoHV-1 and 0.978 for BoHV-5. Analysis of Human Splicing Defects Using. This is done by studying the genome draft of related organisms and it allows. Application of Padlock and Selector Probes in Molecular Medicine 117 11. AlleleID can help you determine the species of a bacteria even if you have a partial oligonucleotide sequence of a gene. Enzymatic and Chemical Cleavage Methods to Identify Genetic Variation 9. Stavrou, Adamandia Papachatzopoulou and George P. It is a RT PCR probe that utilizes fluorescence resonance energy transfer (FRET) and TaqMan methods in order to provide an internal positive control (IPC) for real-time PCR assays. Allele-Specic Mutation Detection 15 133 Holger Tönnies Aglaia Athanassiadou, Eleana F. The multiplex real-time PCR had reaction efficiencies of 1.04 for BoHV-1 and 1.08 for BoHV-5. Triple Labeled Oligos-Put Your Fluorescence in Perspective Allele is now distributing to its customers the newest technology in RT-PCR published less than two weeks ago. The detection limits of the duplex assay were 10 copies of BoHV-1 and 45 copies of BoHV-5. BoHV-1 and BoHV-5 were also correctly identified when incorporated in spiked semen and brain tissue samples.
Pseudomonas aeruginosa (ATCC 1997), and Allele ID (v5.0 Premier Biosoft. The assay correctly identified 22 BoHV-1 and six BoHV-5 isolates from the Biosecurity Sciences Laboratory virus collection. It was successful in iden- TaqMan-MGB probe tifying and enumerating P. allele AllTests Alpha Biosciences Alphabioregen Amoytop Biotech Anogen AnyGenes Ape Apexbio Arbor Assays arista Arthus Biosystems Articular Engineering Artron AS ONE INTERNATIONAL. The multiplex real-time PCR combines a duplex real-time PCR that targets the DNA polymerase gene of BoHV-1 and BoHV-5 and a real-time PCR targeting mitochondrial DNA, as a house-keeping gene, described previously by Cawthraw et al.
A multiplex real-time PCR was developed for the detection and differentiation of two closely related bovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5).
0 kommentar(er)
